Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model
Identifieur interne : 003357 ( Main/Exploration ); précédent : 003356; suivant : 003358Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model
Auteurs : F. Rambow [France] ; O. Malek [France, République tchèque] ; C. Geffrotin [France] ; J. Leplat [France] ; S. Bouet [France] ; G. Piton [France] ; K. Hugot [France] ; C. Bevilacqua [France] ; V. Horak [République tchèque] ; S. Vincent-Naulleau [France]Source :
- Pigment Cell & Melanoma Research [ 1755-1471 ] ; 2008-04.
Abstract
Partial and some few cases of complete spontaneous regression have been observed in cutaneous melanoma patients but little is known about the molecular mechanisms involved. The Melanoblastoma‐bearing Libechov Minipig (MeLiM) is a suitable animal model to study the phenomenon of spontaneous regression because MeLiM pigs exhibit naturally occurring melanomas which regress completely 6 months after birth. In this study, we used suppression subtractive hybridization (SSH) to identify molecular determinants of melanoma regression within swine melanoma tissues and melanoma cell cultures. Several markers involved in cell‐adhesion, ‐communication, ‐motility, signal transduction, negative regulation of cell proliferation, transport and immune response were identified that correlated with melanoma regression whereas the main genes involved in melanin synthesis showed a strong downregulation. For the most differentially expressed genes, we validated the results obtained by SSH with qRT‐PCR and with immunohistochemistry for some of them (CD9, MITF, RARRES1). Most notable, for the first time in melanoma, we identified the retinoic acid responder 1 gene (RARRES1) as a main actor of the regression process in melanoma. This first gene expression study in swine melanoma regression, may contribute to the finding of new therapeutic targets for human melanoma treatment.
Url:
DOI: 10.1111/j.1755-148X.2008.00442.x
Affiliations:
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The Melanoblastoma‐bearing Libechov Minipig (MeLiM) is a suitable animal model to study the phenomenon of spontaneous regression because MeLiM pigs exhibit naturally occurring melanomas which regress completely 6 months after birth. In this study, we used suppression subtractive hybridization (SSH) to identify molecular determinants of melanoma regression within swine melanoma tissues and melanoma cell cultures. Several markers involved in cell‐adhesion, ‐communication, ‐motility, signal transduction, negative regulation of cell proliferation, transport and immune response were identified that correlated with melanoma regression whereas the main genes involved in melanin synthesis showed a strong downregulation. For the most differentially expressed genes, we validated the results obtained by SSH with qRT‐PCR and with immunohistochemistry for some of them (CD9, MITF, RARRES1). Most notable, for the first time in melanoma, we identified the retinoic acid responder 1 gene (RARRES1) as a main actor of the regression process in melanoma. This first gene expression study in swine melanoma regression, may contribute to the finding of new therapeutic targets for human melanoma treatment.</div></front></TEI><affiliations><list><country><li>France</li><li>République tchèque</li></country></list><tree><country name="France"><noRegion><name sortKey="Rambow, F" sort="Rambow, F" uniqKey="Rambow F" first="F." last="Rambow">F. Rambow</name></noRegion><name sortKey="Bevilacqua, C" sort="Bevilacqua, C" uniqKey="Bevilacqua C" first="C." last="Bevilacqua">C. Bevilacqua</name><name sortKey="Bouet, S" sort="Bouet, S" uniqKey="Bouet S" first="S." last="Bouet">S. Bouet</name><name sortKey="Geffrotin, C" sort="Geffrotin, C" uniqKey="Geffrotin C" first="C." last="Geffrotin">C. Geffrotin</name><name sortKey="Hugot, K" sort="Hugot, K" uniqKey="Hugot K" first="K." last="Hugot">K. Hugot</name><name sortKey="Leplat, J" sort="Leplat, J" uniqKey="Leplat J" first="J." last="Leplat">J. Leplat</name><name sortKey="Malek, O" sort="Malek, O" uniqKey="Malek O" first="O." last="Malek">O. Malek</name><name sortKey="Piton, G" sort="Piton, G" uniqKey="Piton G" first="G." last="Piton">G. Piton</name><name sortKey="Rambow, F" sort="Rambow, F" uniqKey="Rambow F" first="F." last="Rambow">F. Rambow</name><name sortKey="Vincent Aulleau, S" sort="Vincent Aulleau, S" uniqKey="Vincent Aulleau S" first="S." last="Vincent-Naulleau">S. Vincent-Naulleau</name></country><country name="République tchèque"><noRegion><name sortKey="Malek, O" sort="Malek, O" uniqKey="Malek O" first="O." last="Malek">O. Malek</name></noRegion><name sortKey="Horak, V" sort="Horak, V" uniqKey="Horak V" first="V." last="Horak">V. Horak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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